Effects of moxibustion at different times on prostaglandin and vasopressin levels in uterine tissues of rats with dysmenorrhea due to cold-dampness retention
| Autor: | Xin-hua Li, Xiao-xue Sun, Fei Gao, Xiao-yi Du, Xue-na Li, Li-hong Sun, Yang Chen, Xiao-shun Ma, Yu-lei Liang |
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| Rok vydání: | 2017 |
| Předmět: |
medicine.medical_specialty
Vasopressin medicine.medical_treatment 0211 other engineering and technologies Uterus Prostaglandin 02 engineering and technology Moxibustion 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine 021105 building & construction medicine Prostaglandin E2 030219 obstetrics & reproductive medicine business.industry Radioimmunoassay medicine.anatomical_structure Endocrinology Complementary and alternative medicine chemistry Oxytocin Estradiol benzoate business medicine.drug |
| Zdroj: | Journal of Acupuncture and Tuina Science. 15:250-256 |
| ISSN: | 1993-0399 1672-3597 |
| DOI: | 10.1007/s11726-017-1009-8 |
| Popis: | To observe the effects of moxibustion at different times on prostaglandin E2 (PGE2), prostaglandin F2α (PGF2α) and arginine vasopressin (AVP), in the uterine tissues of rats with dysmenorrhea due to cold-dampness retention, and to explore the differences and possible mechanisms of moxibustion at different times in easing pain in dysmenorrhea due to cold-dampness retention. Forty-three female Wistar rats were randomly divided into a blank control group (n=7), a model group (n=9), a pre-moxibustion group (n=9), an immediate-moxibustion group (n=9) and a pre-moxibustion plus immediatemoxibustion group (n=9). Rat models of primary dysmenorrhea due to cold-dampness retention were established using (0±1) °C ice water immersion method combined with injection of estradiol benzoate for 10 d, followed by injection of oxytocin on the 11th day. Rats in the 3 intervention groups received moxibustion to Shenque (CV 8) and Guanyuan (CV 4), 10 min for each acupoint, once a day. Rats in pre-moxibustion group were given mild moxibustion, beginning on the 8th day during modeling, for 3 continuous days; rats in immediate-moxibustion group were given one time mild moxibustion, immediately after injection of oxytocin on the 11th day during modeling; rats in pre-moxibustion plus immediate-moxibustion group were given mild moxibustion, beginning on the 8th day during modeling till immediately after injection of oxytocin on the 11th day during modeling, for 4 continuous days. The level of PGF2α in the rat uterine tissues was measured by enzyme-linked immunosorbent assay (ELISA), and the levels of PGE2 and AVP in rat uterine tissues were measured by radioimmunoassay. Compared with the blank control group, the levels of PGF2α and AVP, the PGF2α/PGE2 ratio in the model group were significantly increased (P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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