Deoxyribonuclease I treatment of histones for the detection of anti-histone antibodies in solid-phase immunoassays

Autor:	M. E. Debbas, Marc Monestier, Patel K, Thomas M. Fasy
Rok vydání:	1990
Předmět:	Antigenicity Chymotrypsin Protease biology medicine.diagnostic_test Proteolysis medicine.medical_treatment Immunology Molecular biology Histone Biochemistry Histone H1 Anti-histone antibodies biology.protein medicine Immunology and Allergy Deoxyribonuclease I
Zdroj:	Journal of Immunological Methods. 127:289-291
ISSN:	0022-1759
Popis:	In this report, we tested two commercial preparations of DNase I for their effect on histone H1 and its recognition by a monoclonal anti-H1 antibody. Our results indicate that digestion with the DNase I preparation of a lesser grade results in the complete proteolysis and loss of antigenicity of histone H1 even though this preparation is adversited as containing less than 0.01% chymotrypsin and less than 0.005% protease
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4ca9aab5a57e918aca5c32c55494a2d9 https://doi.org/10.1016/0022-1759(90)90081-6 Zobrazit plný text záznamu