Tear Diagnosis for Diabetic Retinopathy using an Optoelectro-Kinetically Driven Bead-Based Immunosensor
| Autor: | Jen-Yi Wang, Sheng Min Hsu, Jae-Sung Kwon, Han Sheng Chuang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Detection limit
Materials science biology VEGF receptors 010401 analytical chemistry 02 engineering and technology Diabetic retinopathy 021001 nanoscience & nanotechnology medicine.disease 01 natural sciences 0104 chemical sciences Signal enhancement Bead (woodworking) biology.protein medicine Aqueous droplet 0210 nano-technology Laser beams Biomedical engineering |
| Zdroj: | 2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems & Eurosensors XXXIII (TRANSDUCERS & EUROSENSORS XXXIII). |
| DOI: | 10.1109/transducers.2019.8808207 |
| Popis: | Bead-based immunosensing is a promising technology in the point-of-care diagnosis due to its high flexibility. To realize rapid and selective diagnosis, an optoelectrokinetic platyeform, termed rapid electrokinetic patterning (REP), was employed herein to drive a bead-based fluorescent immunosensor for diagnosis of diabetic retinopathy by lipocalin 1 (LCN1) and vascular epithelial growth factor (VEGF). The measurement was made in an aqueous droplet sandwiched between two parallel electrodes. With an electric field and a focused laser beam applying on the microchip simultaneously, the immunocomplexes in the droplet were further concentrated to enhance the fluorescent signal. The limit of detection (LOD) reached nearly 110 pg/mL. A DR phase plot was made to provide a clear way to determine whether a tested subject was under the threat of DR. At last, a preclinical evaluation was conducted. The accuracy of the proposed technique reached 94.74% in PDR and 89.47% in DM. The optoelectrokinetically driven bead-based immunosensor enables a noninvasive measure to the early diagnosis of diabetic retinopathy. |
| Databáze: | OpenAIRE |
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