Toxicity of TiO2 nanoparticles on the NRK52E renal cell line
| Autor: | Sophie Laurent, Alexandre Robbe, Alexandre Legrand, Xavier Valentini, Lara Absil, Denis Nonclercq, Robert N. Muller, Guy Laurent |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
chemistry.chemical_classification Reactive oxygen species Health Toxicology and Mutagenesis Public Health Environmental and Occupational Health Nanoparticle 010501 environmental sciences Toxicology 01 natural sciences In vitro Pathology and Forensic Medicine 03 medical and health sciences 030104 developmental biology chemistry Dynamic light scattering Apoptosis Cell culture Toxicity Biophysics Turbidimetry General Pharmacology Toxicology and Pharmaceutics 0105 earth and related environmental sciences |
| Zdroj: | Molecular & Cellular Toxicology. 13:419-431 |
| ISSN: | 2092-8467 1738-642X |
| DOI: | 10.1007/s13273-017-0046-1 |
| Popis: | Titanium dioxide nanoparticles are widely used in industry to produce a number of products (cosmetics, paints, foods). TiO2 nanoparticles form aggregates and their toxicity varies in function of their size. In this study, aggregate sizes were determined using dynamic light scattering (DLS). The stability of the suspension was evaluated by turbidimetry and the size by transmission electronic microscopy (TEM). In the present study the toxicity of TiO2 nanoparticles was evaluated in vitro on rat kidney proximal tubular cells (NRK-52E). Cells were exposed to TiO2 nanoparticles (20 μg/mL) for 24, 48, 72 and 96 hours. TiO2 nanoparticles induce the production of reactive oxygen species (ROS). The relative number of mitosis decreased, while an increase of apoptotic cells was noted. The number of S-phase cells evidenced by BrdU immunoreactivity in cultures was significantly reduced by the exposure to the nanoparticles. These results attest to the toxic effect of the TiO2 nanoparticles on NRK-52E cells. |
| Databáze: | OpenAIRE |
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