Synergy of a virulent phage (φAB182) with antibiotics leading to successful elimination of biofilms formed by MDR Acinetobacter baumannii

Autor:	Medhavi Vashisth, Shikha Yashveer, Anu Bala Jaglan, Nitin Virmani, Bidhan Chand Bera, Rajesh Kumar Vaid, Taruna Anand
Rok vydání:	2022
Předmět:	Immunology Genetics General Medicine Molecular Biology Applied Microbiology and Biotechnology Microbiology
Zdroj:	Canadian Journal of Microbiology. 68:731-746
ISSN:	1480-3275 0008-4166
DOI:	10.1139/cjm-2022-0080
Popis:	Emergence of multiple drug resistant (MDR) strains of Acinetobacter baumannii and a withering drug discovery pipeline necessitates the search for effective alternatives to replace or synergize with currently used antibiotics. In this report, we have described the synergy assessment of a virulent Acinetobacter baumannii phage φAB182 with a wide range of antibiotics. Myophage φAB182 was isolated from sewage against MDR A. baumannii and exhibited maximum stability at 25 °C and pH 7. It also had a short latent period of 9 min with a large burst size of 287. The phylogenetic analysis of its major capsid protein gene indicated an 84.15% similarity to the lytic A. baumannii phage Acj9. In the presence of antibiotics, phage φAB182 showed the highest synergy ( p combination/MBECantibiotic values (
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4c9e0cb02cc70cece185375966de3a14 https://doi.org/10.1139/cjm-2022-0080 Zobrazit plný text záznamu Plný text ve formátu PDF