Effect of nitric oxide synthase inhibition onSchistosoma japonicumegg-induced granuloma formation in the mouse liver
| Autor: | Kazuho Hirata, Mizuki Hirata, Toshihide Fukuma, Tatsuru Hara, Masayoshi Kage, Mei Zhang |
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| Rok vydání: | 2001 |
| Předmět: |
Granuloma formation
Schistosoma japonicum egg biology medicine.medical_treatment Schistosoma japonicum Immunology medicine.disease biology.organism_classification Nitric oxide Nitric oxide synthase chemistry.chemical_compound Cytokine chemistry Fibrosis Granuloma medicine biology.protein Parasitology |
| Zdroj: | Parasite Immunology. 23:281-289 |
| ISSN: | 0141-9838 |
| DOI: | 10.1046/j.1365-3024.2001.00384.x |
| Popis: | Nitric oxide (NO) plays diverse roles in a variety of pathological processes. We investigated the role of NO in Schistosoma japonicum egg-induced granuloma formation in a mouse hepatic model. Immunohistological analysis revealed that there is the most intense and extensive inducible nitric oxide (iNOS) expression 2 weeks after egg implantation, and thereafter it decreased considerably with time. Treatment with nitric oxide synthase inhibitors, NIL (lN6- (iminoethyl)-lysine) or Nω-nitro-l-arginine methyl ester (l-NAME), resulted in two different types of unusual granulomas at 2 weeks. One type showed suppressed fibrosis, while another showed foreign body-type multinuclear cell formation which frequently appeared particularly when 50 µg/ml NIL was given. At 3 weeks following treatment, fibrotic granulomas with scanty peripheral cellularity was obvious. However, there were no apparent changes after this period (at 4 weeks). Cytokine analysis in NIL-treated mice showed a significant increase of IL-4 and IL-13 production at 2 weeks. These findings indicated that nitric oxide contributes to granuloma development during the early stages, probably through the regulation of Th2 cytokine production. |
| Databáze: | OpenAIRE |
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