Effect of Garlic on Rat Diabetic Renal Cortex. A histological and Immunohistochemical Study
| Autor: | Omnia S. Erfan |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
medicine.medical_specialty
biology business.industry Renal cortex food and beverages Vimentin Haematoxylin medicine.disease Streptozotocin Diabetic nephropathy chemistry.chemical_compound medicine.anatomical_structure Endocrinology chemistry Internal medicine Diabetes mellitus biology.protein Medicine Immunohistochemistry Desmin business medicine.drug |
| Zdroj: | The Egyptian Journal of Anatomy. 40:253-264 |
| ISSN: | 2090-259X |
| DOI: | 10.21608/ejana.2018.16904 |
| Popis: | Background: Diabetic nephropathy is one of the chief microvascular complications of diabetes and it has turn into the main cause of end-stage renal disease nictitate dialysis.Aim of work: To observe the effects of garlic supplementation on STZ induced diabetic nephropathy in Albino rats using histological and immunohistochemichal stains.Material and Methods: Three groups were included, control group of 5 rats served as control, experimental group of streptozotocin- induced diabetes with dose of 50 mg/kg body weight intraperitoneal for eight weeks and third group is the diabetic rats supplemented with garlic orally with a dose of (500 mg/kg body weight) for 6 weeks starting two weeks after diabetes induction.At the end of the experiment, rats were sacrificed then kidneys were dissected out and processed for paraffin blocks. Sections were stained with haematoxylin & eosin and immunohistochemistry for vimentin, nuclear factor kappa and desmin.Results: Increased diameter of glumeruli in diabetic rats. These changes were decreased in the diabetic group treated with garlic. Desmin, vimentin and NF-κB expression were induced in diabetic group but they decreased after garlic supplementation.Conclusion: The changes in diabetic group were regressed with garlic supplementation. It is advisable to give natural products like garlic to diabetic patients as they help to ameliorate diabetic complications. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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