Sequence-specific assignment of the backbone1H- and31P-nmr lines in a short DNA duplex with homo- and heteronuclear correlated spectroscopy
| Autor: | M. H. Frey, W. Leupin, O. W. Sørensen, W. A. Denny, R. R. Ernst, K. Wüthrich |
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| Rok vydání: | 1985 |
| Předmět: |
Dna duplex
Chemistry Organic Chemistry Biophysics General Medicine Nuclear magnetic resonance spectroscopy Biochemistry Biomaterials chemistry.chemical_compound Crystallography Nuclear magnetic resonance Heteronuclear molecule Duplex (building) Double quantum Spectroscopy Two-dimensional nuclear magnetic resonance spectroscopy DNA |
| Zdroj: | Biopolymers. 24:2371-2380 |
| ISSN: | 0006-3525 |
| DOI: | 10.1002/bip.360241214 |
| Popis: | We report the assignment of the backbone 1H- and 31P-nmr lines in the synthetic hexadeoxyribonucleotide pentaphosphate duplex d(GCATGC)2, using double quantum filtered 1H-1H correlation spectroscopy, 1H observed 1H-31P heteronuclear correlation spectroscopy, and 31P relayed 1H-1H correlation spectroscopy. The strategy used enables one to make sequence-specific resonance assignments without reference to a known or assumed conformation of the DNA fragment. |
| Databáze: | OpenAIRE |
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