Identification and functional characterization of Penicillium marneffei major facilitator superfamily (MFS) transporters
| Autor: | Xinyue Chen, Takashi Yaguchi, Anom Bowolaksono, Susumu Kajiwara, Setyowati Triastuti Utami, Masakazu Niimi, Carissa Ista Indriani, Kyoko Niimi |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
030106 microbiology Organic Chemistry Transporter General Medicine Biology medicine.disease biology.organism_classification Applied Microbiology and Biotechnology Biochemistry Major facilitator superfamily Analytical Chemistry Microbiology Southeast asia Multiple drug resistance 03 medical and health sciences Penicilliosis 030104 developmental biology Thermally dimorphic fungus medicine Identification (biology) Penicillium marneffei Molecular Biology Biotechnology |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 84:1373-1383 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1080/09168451.2020.1732185 |
| Popis: | Penicillium marneffei is a thermally dimorphic fungus that causes penicilliosis, and become the third-most-common opportunistic fungal infection in immunocompromised patients in Southeast Asia. Azoles and amphotericin B have been introduced for the treatment, however, it is important to investigate possible mechanisms of azole resistance for future treatment failure. We identified 177 putative MFS transporters and classified into 17 subfamilies. Among those, members of the Drug:H+ antiporter 1 subfamily are known to confer resistance to antifungals. Out of 39 paralogs, three (encoded by PmMDR1, PmMDR2, and PmMDR3) were heterologously overexpressed in S. cerevisiae AD∆ conferred resistance to various drugs and compounds including azoles, albeit to different degrees. PmMDR1-expressing strain showed resistance to the broadest range of drugs, followed by the PmMDR3, and PmMDR2 conferred weak resistance to a limited range of drugs. We conclude that PmMDR1 and PmMDR3, may be able to serve as multidrug efflux pumps. |
| Databáze: | OpenAIRE |
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