Two-Photon Excitation of Fluorogenic Probes for Redox Metabolism: Dramatic Enhancement of Optical Contrast Ratio by Two-Photon Excitation
| Autor: | Vojtech Balsanek, Qingdong Zheng, Dalibor Sames, Paras N. Prasad, Steffen Jockusch, Nicholas J. Turro, Guang S. He, Dominic J. Yee, Haridas E. Pudavar, Marlin Halim |
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| Rok vydání: | 2007 |
| Předmět: |
Molecular switch
Chemistry Analytical chemistry Photochemistry Laser Fluorescence Surfaces Coatings and Films Electronic Optical and Magnetic Materials law.invention General Energy Two-photon excitation microscopy law Femtosecond Contrast ratio Physical and Theoretical Chemistry Absorption (electromagnetic radiation) Excitation |
| Zdroj: | The Journal of Physical Chemistry C. 111:8872-8877 |
| ISSN: | 1932-7455 1932-7447 |
| DOI: | 10.1021/jp067430h |
| Popis: | Fluorogenic probes for redox metabolism are shown to exhibit two-photon absorption using femtosecond pulses from a Ti/sapphire laser. The probes consist of a coumarin or naphthalene core and a ketone−alcohol functional group. The probe design is based on the changes in fluorescence properties and two-photon cross section values when the ketone derivative is reduced to the corresponding alcohol. The resulting contrast ratio of the fluorescence differs significantly from that obtained by one-photon excitation. This phenomenon was demonstrated with all three switches examined herein and represents an attractive approach to modulation of emission properties of molecular switches. The practical applicability of the two-photon-excitation redox switch was demonstrated in an enzyme-catalyzed transformation. The nonfluorescent probe 1 is efficiently converted by AKR1C3, a human 3α-hydroxysteroid dehydrogenase, to the fluorescent derivative 2. Using two-photon excitation (775 nm), we achieved a large contrast ratio... |
| Databáze: | OpenAIRE |
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