| Popis: |
The impact of Salicylic acid (SA) and Methyl jasmonate (MJ) on growth, and essential oil quantity and quality in organ culture of Acinos graveolens were investigated. In the present study, nodal segments were used as explants for shoot production using MS media with BA and NAA. Afterward, they were transferred to liquid MS media culture containing SA and MJ with 50, 100, and 150 µM and their combinations (SA25+MJ25, SA50+MJ25, SA25+MJ50, and SA50+MJ50). After three weeks, samples were collected to assess the morphological and some growth parameters, quantitative changes in pigment amounts as well as volatile contents. Accordingly, almost all treatments led to a notable decrease in growth parameters in comparison with control. The highest amount of Chl a, b and carotenoids were achieved by MJ100, SA50, and SA25+MJ50, respectively. In addition, GC-MS results indicated 38 components of volatile products mainly alkane hydrocarbons and sesquiterpenes. The major identified compounds were Decane, Eicosane, and Germacrene D. Altogether, results asserted that SA25+MJ25 and SA100 were more efficient in the enhancement of essential oil content among all treatments. |
