Synthesis of Bis-piperazine-type pH Buffer Agents and Their Application to Mammalian Cell Cultures
| Autor: | Kenyu Kina, Hiroki Murakami, Masanobu Shiga, Kazuhiko Nagira, Kazumi Sasamoto, Midori Hayashida, Takuya Sugahara |
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| Rok vydání: | 1994 |
| Předmět: |
HEPES
Chromatography Chemistry Cell growth Organic Chemistry General Medicine Buffer solution Applied Microbiology and Biotechnology Biochemistry Chemical synthesis Buffer (optical fiber) Analytical Chemistry Piperazine chemistry.chemical_compound Cell culture Ethanesulfonic acid Molecular Biology Biotechnology Nuclear chemistry |
| Zdroj: | Bioscience, Biotechnology, and Biochemistry. 58:1370-1375 |
| ISSN: | 1347-6947 0916-8451 |
| DOI: | 10.1271/bbb.58.1370 |
| Popis: | New bis-piperazine-type pH buffer agents were synthesized and their buffering properties were evaluated. The compounds proved to have two-fold larger pH buffering ability than 2-[4-(2-hydroxyethyl)-1-piperazinyl]ethanesulfonic acid (HEPES), a Good’s buffer traditionally used to control the pH value of culture media.Human-human hybridoma HB4C5 cells were cultured in a serum-free medium containing these buffer agents. The cell growth and antibody production, using 1,2-N,N′-bis[N′′,N′′′-di(2-sulfonoethyl)piperazinyl]ethane, were greater than when HEPES. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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