Recovery of α-Agarase Enzyme from Fermentation Broths by Membrane Crossflow Filtration

Autor:	G. Toussaint, M. Nonus, I. Hassairi, Michel Y. Jaffrin, L. H. Ding
Rok vydání:	2000
Předmět:	Chromatography biology Chemistry Process Chemistry and Technology General Chemical Engineering Microfiltration Analytical chemistry Filtration and Separation General Chemistry Permeation Enzyme assay Membrane technology Cross-flow filtration Shear rate Membrane Sieving coefficient biology.protein
Zdroj:	Separation Science and Technology. 35:795-809
ISSN:	1520-5754 0149-6395
DOI:	10.1081/ss-100100193
Popis:	We describe the recovery of extracellular α-agarase enzyme from fermentation broths using a polypropylene hollow fiber filter with 0.5 μm pores normally used for plasma collection from donors. The permeate flux increased with inlet wall shear rate from 30 L/h·m2 at 1500 s−1 to 45 L/h·m2 at 3000 s−1. But the enzyme activity sieving coefficient (ratio of permeate to inlet activity) decreased with increasing shear rate and transmembrane pressure (TMP). Therefore, the enzyme flux transmitted was found to be maximum at a shear rate of 1500 s−1 and a TMP between 20 and 40 kPa. Since enzyme activity decay with time is less at low temperature, the recovered activity was found to be nearly independent of temperature between 5 and 37°C, the maximum occurring at 22°C. The superimposition of flow and pressure pulsations on the inlet and outlet lines of the filter with a modified roller pump produced permeate flux enhancements ranging from 44% at 1000 s−1 to 68% at 3000 s−1 for a TMP of 3 kPa.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4c10f86236199b979c51d9fa8133c4aa https://doi.org/10.1081/ss-100100193 Zobrazit plný text záznamu