Fluid phase endocytosis in oral epithelia: variation with site and effect of cancer
| Autor: | A. H. Saleh, W. Hall, Graham R. Ogden, P. E. Ross, D. V. Hopwood |
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| Rok vydání: | 2000 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty medicine.diagnostic_test business.industry Organ culture Endocytosis Epithelium Pathology and Forensic Medicine law.invention Flow cytometry stomatognathic diseases medicine.anatomical_structure Otorhinolaryngology Epidermoid carcinoma Confocal microscopy law Biopsy medicine Periodontics Oral Surgery Oral mucosa business |
| Zdroj: | Journal of Oral Pathology & Medicine. 29:220-225 |
| ISSN: | 0904-2512 |
| DOI: | 10.1034/j.1600-0714.2000.290505.x |
| Popis: | The structure of the oral mucosa has been extensively studied but its cell physiology has been less well characterised. This study aimed to show the range in variation in fluid phase endocytic capability in biopsies from different oral sites. Oral epithelial cells were obtained from both biopsies and single-cell suspensions obtained by brushing the oral cavity. Biopsies in organ culture and single cells in suspension were incubated with fluorescent microspheres of 0.02, 0.1 or 1.0 microm diameter. Endocytosis of fluorescent microspheres was quantitated by flow cytometry and visualised by confocal microscopy. Epithelial cells from all oral sites that were sampled internalised 0.02 microm and 0.1 microm but not 1.0 microm microspheres, with no significant differences observed between oral regions. Single cells from non-cancer patients endocytosed significantly more 0.02 microm microspheres than cells removed from patients with oral cancer. This model may be used to study integrated oral cell function both in health and disease. |
| Databáze: | OpenAIRE |
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