Computer-Aided Planning for the Verification of Large Batches of DNA Constructs
| Autor: | Valentin Zulkower |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
chemistry.chemical_classification Sanger sequencing 0303 health sciences Computer science Computational biology 01 natural sciences 03 medical and health sciences Synthetic biology Restriction enzyme symbols.namesake chemistry.chemical_compound Enzyme chemistry 010608 biotechnology symbols Computer-aided Restriction digest DNA construct Primer (molecular biology) DNA 030304 developmental biology |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071610312 |
| Popis: | Restriction digest analysis and Sanger sequencing are among the most commonly used techniques to check the sequence of synthetic DNA constructs. However, both require careful preparation to select restriction enzymes or DNA primers adapted to the expected constructs sequences. In projects involving manufacturing of large batches of synthetic constructs, the task can be tedious and error-prone. This chapter demonstrates the use of two free and open-source web applications providing fast and automated selection of enzymes and sequencing primers for DNA construct verification. |
| Databáze: | OpenAIRE |
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