Single‐cell dissection and microdroplet chemistry

Autor:	Shuqiu Zhang, William H. Outlaw
Rok vydání:	2001
Předmět:	Carbon-Carbon Lyases medicine.anatomical_structure Biochemistry Physiology Chemistry Cellular differentiation Cell medicine Plant Science Organism Isolated cell
Zdroj:	Journal of Experimental Botany. 52:605-614
ISSN:	1460-2431 0022-0957
DOI:	10.1093/jexbot/52.356.605
Popis:	The unique roles of individual cells may be critical to the physiology of an organism. In such cases, micromethods are essential to elucidating the molecular biology, biochemistry and biophysics of the specialized cells or even subcellular compartments of the important cells. The great proliferation of micromethods testifies to their value and no single review can be comprehensive. This review therefore provides only a generalized overview of one approach, namely dissection that provides a pure sample for subsequent extraction and analysis by microdroplet chemistry. As a means of illustrating the utility of this approach, an application-study of the interaction of cytosolic malate concentration and guard-cell phosphoenolpyruvate carboxylase-is provided.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4bfb3a13a103efc89bfedfc94c9e23dc https://doi.org/10.1093/jexbot/52.356.605 Zobrazit plný text záznamu