Photoinduced production of reactive oxygen species by retinal derivatives and conjugates

Autor:	T. F. Shevchenko, G. R. Kalamkarov, P. V. Aboltin, Konstantin B. Shumaev
Rok vydání:	2013
Předmět:	chemistry.chemical_classification Reactive oxygen species Superoxide Biophysics Albumin Quantum yield Retinal Photochemistry Adduct law.invention chemistry.chemical_compound chemistry law Hydroxyl radical Electron paramagnetic resonance
Zdroj:	Biophysics. 58:178-182
ISSN:	1555-6654 0006-3509
DOI:	10.1134/s0006350913020024
Popis:	Formation of reactive oxygen species (ROS) during all-trans-retinal (ATR) illumination was studied by EPR. The quantity of ROS was estimated from the EPR signal of a spin adduct of DEPMPO (5-diethoxyphosphoryl-5-methyl-1-pyrroline-N-oxide). The EPR signal was found to be a superposition of the adducts: 80% superoxide anion radical, 15% hydroxyl radical, and 5% unknown radical. Albumin at an equimolar concentration decreased the relative quantum yield of ROS five times, and a 4-fold albumin excess decreased ROS production 30 times. It is supposed that ATR and A2E in the photoreceptor membrane are the sources of photodamage induced by ROS. Since ATR in the cell is known to be transported by interphotoreceptor retinal-binding proteins of the albumin family, its binding to protein is supposed to play an important protective role, preventing ROS production in the photoreceptor cell.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4bf384b55b8e84bc4cb6bb72c6796095 https://doi.org/10.1134/s0006350913020024 Zobrazit plný text záznamu Full text from SpringerLink