Single pass sequencing and physical and genetic mapping of human brain cDNAs

Autor:	Andrea S. Wilcox, Arto K. Orpana, Misi Robinson, Akbar S. Khan, James M. Sikela, Janet A. Hopkins, Mihael H. Polymeropoulos, T.J. Stevens
Rok vydání:	1992
Předmět:	Untranslated region Genetics Single pass medicine.anatomical_structure Gene mapping Genetic marker Complementary DNA medicine Human genome Human brain Biology
Zdroj:	Nature Genetics. 2:180-185
ISSN:	1546-1718 1061-4036
DOI:	10.1038/ng1192-180
Popis:	We have performed single pass sequencing of 1,024 human brain cDNAs, over 900 of which seem to represent new human genes. Library prescreening with total brain cDNA significantly reduced repeated sequencing of highly represented cDNAs. A subset of sequenced cDNAs were physically mapped to their chromosomal locations using gene–specific STS primers derived from 3′ untranslated regions. We have also determined that human brain cDNAs represent a rich source of gene–associated polymorphic markers. Microsatellite–containing cDNAs can be physically mapped and converted to highly informative genetic markers, thus facilitating integration of the human physical, expression and genetic maps.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4bef67b2f6e4930019c752581843cb55 https://doi.org/10.1038/ng1192-180 Zobrazit plný text záznamu