| Autor: |
J. Roper, P.G. Jenkins, J. E. Harris, J.V. Hone, A. B. Wrathmell, T.H. Grayson, P.M. Russell |
| Rok vydání: |
1995 |
| Předmět: |
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| Zdroj: |
Aquaculture. 132:221-232 |
| ISSN: |
0044-8486 |
| DOI: |
10.1016/0044-8486(94)00402-a |
| Popis: |
Vaccination is potentially one of the most valuable methods of control against the caligid copepod Lepeophtheirus salmonis , the salmon louse, a major pathogen of farmed salmonids in the Northern hemisphere. A search for suitable potential antigens from this complex metazoan parasite forms the basis of this study. Salmon louse homogenate was separated by column chromatography into specific fractions which were assayed for a range of enzyme activities. Fractions found to be associated with enzymic activity or peaks of UV absorbance were used to raise antisera in 44 Wistar rats. An immunocytochemical investigation of the midgut of the adult louse using these specific antisera revealed immunostaining of the lumenal and basal surfaces of the louse gut. It is suggested that the proteins in the chromatographic fractions may represent digestive enzymes and/or structural or functional components of the salmon louse gut. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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Full Text from ScienceDirect