Transduction of mouse retina by insect cell packaged recombinant adeno-associated viruses and their mutants via intravitreal injection

Autor:	Jianxiang Wu, Qungang Zhou, Xiaomei Liu, Taiming Li, Chun Zhang, Wei Zheng, Xiaofang Li
Rok vydání:	2021
Předmět:	0303 health sciences Insect cell viruses Mutant Biology eye diseases Cell biology law.invention 03 medical and health sciences Transduction (genetics) 0302 clinical medicine Mouse Retina law Virology 030221 ophthalmology & optometry Recombinant DNA sense organs 030304 developmental biology
Zdroj:	Future Virology. 16:181-190
ISSN:	1746-0808 1746-0794
Popis:	Aim: Adeno-associated virus (AAV) is the most preferred gene therapy vector. The purpose of our research is to compare the infection tropism and gene expression efficiency of vitreous injection of recombinant AAVs (rAAVs) and their capsid mutants in mouse retina. Materials & methods: We packaged wild-type rAAV2/2,6,8,9 and their capsid mutants carrying EGFP expression cassette using insect cells. The gene expression profiles of rAAVs and their mutants in mouse retina were evaluated by optical imaging of retinal tissue flat mount and cryosections. Results & conclusion: The results showed that rAAV2 and rAAV2-Y444F mainly targeted retinal ganglion cell; rAAV8, rAAV8-Y733F, rAAV9 and mutants had obvious EGFP expression in retinal pigment epithelium cells. Compared with the wild-type rAAVs, capsid mutants have an improved transduction efficiency in mouse retina cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4bbee5940233a49c287371eafdf0c757 https://doi.org/10.2217/fvl-2020-0096 Zobrazit plný text záznamu