| Autor: | James C. Wallace, Sean Proll, D. Lorne Tyrrell, Michael A. Joyce, Jeff Furlong, Jill C. Thompson, Michael G. Katze, Maria W. Smith, Kathie-Anne Walters |
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| Rok vydání: | 2006 |
| Předmět: |
0303 health sciences
Microarray Hepatitis C virus Genomics Hepatitis C Biology medicine.disease medicine.disease_cause Virology Molecular biology 3. Good health 03 medical and health sciences Nucleic acid thermodynamics 0302 clinical medicine Infectious Diseases 030220 oncology & carcinogenesis Gene expression medicine Gene Functional genomics 030304 developmental biology |
| Zdroj: | Virology Journal. 3:37 |
| ISSN: | 1743-422X |
| DOI: | 10.1186/1743-422x-3-37 |
| Popis: | Background Many model systems of human viral disease involve human-mouse chimeric tissue. One such system is the recently developed SCID-beige/Alb-uPA mouse model of hepatitis C virus (HCV) infection which involves a human-mouse chimeric liver. The use of functional genomics to study HCV infection in these chimeric tissues is complicated by the potential cross-hybridization of mouse mRNA on human oligonucleotide microarrays. To identify genes affected by mouse liver mRNA hybridization, mRNA from identical human liver samples labeled with either Cy3 or Cy5 was compared in the presence and absence of known amounts of mouse liver mRNA labeled in only one dye. |
| Databáze: | OpenAIRE |
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