Pathological characteristics of Ccdc85c knockout rats: a rat model of genetic hydrocephalus
| Autor: | Natsuki Tanaka, Mitsuru Kuwamura, Takeshi Izawa, Tetsushi Sakuma, Takashi Yamamoto, Shizuka Konishi, Takehito Kaneko, Jyoji Yamate, Tomoji Mashimo, Miyuu Tanaka |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Knockout rat General Veterinary Effector Cerebrum General Medicine Biology medicine.disease Phenotype General Biochemistry Genetics and Molecular Biology Hydrocephalus 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Transcription (biology) medicine Animal Science and Zoology Gene Pathological 030217 neurology & neurosurgery |
| Zdroj: | Experimental Animals. 69:26-33 |
| ISSN: | 1881-7122 1341-1357 |
| Popis: | Spontaneous hhy mice show hydrocephalus and subcortical heterotopia, and a mutation in the Ccdc85c gene has been identified. To contribute to the comparison of the role of Ccdc85c in different species, we established a Ccdc85c KO rat and investigated its pathological phenotypes. Ccdc85c KO rats were produced by genomic engineering using transcription activator-like effector nuclease (TALEN). The KO rats had an approximately 350-bp deletion in Ccdc85c and lacked CCDC85C protein expression. The KO rats showed non-obstructive hydrocephalus, subcortical heterotopia, and intracranial hemorrhage. The KO rats had many pathological characteristics similar to those in hhy mice. These results indicate that CCDC85C plays an important role in cerebral development in rats, and the function of CCDC85C in the cerebrum are similar in rats and mice. |
| Databáze: | OpenAIRE |
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