Ionic liquid-based microwave-assisted extraction and HPLC analysis of dehydrocavidine incorydalis saxicolaBunting
| Autor: | F.-Y. Du, G.-K. Li, P.-P. Xu, X.-H. Xiao |
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| Rok vydání: | 2010 |
| Předmět: | |
| Zdroj: | Acta Chromatographica. 22:459-471 |
| ISSN: | 2083-5736 1233-2356 |
| Popis: | Summary An efficient ionic liquid-based microwave-assisted (IL-MAE) method has been developed for extraction of dehydrocavidine from Corydalis saxicola Bunting (C. saxicola) for subsequent rapid analysis by high-performance liquid chromatography (HPLC). The yield of dehydrocavidine reached 9.446 mg g−1 within 10 min under the optimum IL-MAE conditions (1.5 mol L−1 [hmim]Br as extraction solvent, liquid-to-solid ratio 20:1 (mL:g), and extraction temperature 70°C). Compared with conventional procedures, the proposed IL-MAE method has many advantages, for example high extraction yield, short extraction time, low solvent consumption, no use of volatile organic solvents, and no further sample clean-up before HPLC analysis. The method was validated for limit of detection (LOD) and quantification (LOQ), linearity, precision, recovery, and reproducibility. The calibration range was 5.0–200 mg L−1 and the correlation coefficient, r, was 0.9996. The LOD and LOQ were 0.035 and 0.12 mg L−1, respectively. The relative... |
| Databáze: | OpenAIRE |
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