Binding of the Atg1/ULK1 kinase to the ubiquitin-like protein Atg8 regulates autophagy
| Autor: | Isabella Hansmann, Claudine Kraft, Matthias Peter, Andrea Brezovich, Edyta Siergiejuk, Monika Kijanska, Sung Sik Lee, Ingrid Stoffel, Kay Hofmann, Eyal Kalie, Gustav Ammerer, Sharon A. Tooze, Giuseppe Semplicio, Mayanka Verma |
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| Rok vydání: | 2012 |
| Předmět: |
0303 health sciences
Autophagy-Related Protein 8 Family Atg1 General Immunology and Microbiology General Neuroscience ATG8 Autophagosome maturation 030302 biochemistry & molecular biology Autophagy mTORC1 Autophagy-related protein 13 Biology General Biochemistry Genetics and Molecular Biology Cell biology 03 medical and health sciences Autophagy-Related Protein-1 Homolog Molecular Biology 030304 developmental biology |
| Zdroj: | The EMBO Journal. 31:3691-3703 |
| ISSN: | 0261-4189 |
| DOI: | 10.1038/emboj.2012.225 |
| Popis: | Autophagy is an intracellular trafficking pathway sequestering cytoplasm and delivering excess and damaged cargo to the vacuole for degradation. The Atg1/ULK1 kinase is an essential component of the core autophagy machinery possibly activated by binding to Atg13 upon starvation. Indeed, we found that Atg13 directly binds Atg1, and specific Atg13 mutations abolishing this interaction interfere with Atg1 function in vivo . Surprisingly, Atg13 binding to Atg1 is constitutive and not altered by nutrient conditions or treatment with the Target of rapamycin complex 1 (TORC1)‐inhibitor rapamycin. We identify Atg8 as a novel regulator of Atg1/ULK1, which directly binds Atg1/ULK1 in a LC3‐interaction region (LIR)‐dependent manner. Molecular analysis revealed that Atg13 and Atg8 cooperate at different steps to regulate Atg1 function. Atg8 targets Atg1/ULK1 to autophagosomes, where it may promote autophagosome maturation and/or fusion with vacuoles/lysosomes. Moreover, Atg8 binding triggers vacuolar degradation of the Atg1–Atg13 complex in yeast, thereby coupling Atg1 activity to autophagic flux. Together, these findings define a conserved step in autophagy regulation in yeast and mammals and expand the known functions of LIR‐dependent Atg8 targets to include spatial regulation of the Atg1/ULK1 kinase. |
| Databáze: | OpenAIRE |
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