Isolation of paraquat-resistant mutants ofEscherichia coli: lack of correlation between resistance and the activity of superoxide dismutase
| Autor: | Su Mei Kao, Hosni M. Hassan |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
inorganic chemicals
biology Superoxide Mutant Wild type medicine.disease_cause biology.organism_classification Microbiology Enterobacteriaceae Superoxide dismutase chemistry.chemical_compound chemistry Paraquat Catalase Genetics biology.protein medicine heterocyclic compounds Molecular Biology Escherichia coli |
| Zdroj: | FEMS Microbiology Letters. 28:93-97 |
| ISSN: | 1574-6968 0378-1097 |
| DOI: | 10.1111/j.1574-6968.1985.tb00771.x |
| Popis: | Paraquat-resistant Escherichia coli mutants were isolated. The mutants were 10- to 50-fold more resistant to paraquat than the wild type. The wild type was more responsive to the presence of paraquat by inducing higher levels of the manganese-containing superoxide dismutase (MnSOD). Thus, in minimal medium, 0.1 mM paraquat caused a 5-fold increase in MnSOD in the wild type while it had no effect on the level of MnSOD in the mutants. Yet, 50 mM paraquat exerted a dramatic induction of SOD in the mutant strains when grown in trypticase soy yeast extract (TSY) medium. In TSY medium, catalase was not significantly affected by paraquat in all the strains tested. Resistance to paraquat in these mutant strains is, therefore, unrelated to their capacity to detoxify superoxide or hydrogen peroxide. |
| Databáze: | OpenAIRE |
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