ToF-SIMS as a tool for metabolic profiling small biomolecules in cancer systems
| Autor: | Nicholas P. Lockyer, John S. Fletcher, John C. Vickerman, Alex Henderson, Helen L. Kotze, Kaye J. Williams, Emily G. Armitage |
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| Rok vydání: | 2012 |
| Předmět: |
chemistry.chemical_classification
Biomolecule Metabolite Surfaces and Interfaces General Chemistry Computational biology Condensed Matter Physics Surfaces Coatings and Films Secondary ion mass spectrometry chemistry.chemical_compound Drug treatment Metabolomics Nuclear magnetic resonance chemistry Potential biomarkers Principal component analysis Chemical specificity Materials Chemistry |
| Zdroj: | Surface and Interface Analysis. 45:277-281 |
| ISSN: | 0142-2421 |
| Popis: | Time-of-flight secondary ion mass spectrometry (ToF-SIMS) is emerging as a tool for studying the metabolism of disease. ToF-SIMS enables chemical specificity in addition to high spatial resolution imaging of biological samples from cells to tissue. Here, ToF-SIMS has been used to investigate the metabolic regulation of hypoxia-induced chemoresistance to doxorubicin treatment using multicellular tumour spheroids. Imaging principal component analysis (PCA) was used as a tool to identify the regions of chemistry present within the image that differ as a result of drug treatment. A series of metabolite ToF-SIMS spectra were acquired, which were used to identify quasi-molecular ions and fragments correlated to the PCA loading plots. Metabolite patterns have been identified as potential biomarkers of hypoxia-induced chemoresistance. |
| Databáze: | OpenAIRE |
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