Uptake of TiO2 Nanoparticles into C. elegans Neurons Negatively Affects Axonal Growth and Worm Locomotion Behavior
| Autor: | Gong-Her Wu, Tzu-En Hua, Ta-Jen Yen, Oliver I. Wagner, Chun-Chih Hu |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
education.field_of_study Anatase Materials science biology fungi Tio2 nanoparticles Population technology industry and agriculture 02 engineering and technology Body size 021001 nanoscience & nanotechnology biology.organism_classification 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Cytoplasm Titanium dioxide Biophysics General Materials Science Fluorescein 0210 nano-technology education Caenorhabditis elegans |
| Zdroj: | ACS Applied Materials & Interfaces. 10:8485-8495 |
| ISSN: | 1944-8252 1944-8244 |
| Popis: | We employ model organism Caenorhabditis elegans to effectively study the toxicology of anatase and rutile phase titanium dioxide (TiO2) nanoparticles (NPs). The experimental results show that nematode C. elegans can take up fluorescein isothiocyanate-labeled TiO2 NPs and that both anatase and rutile TiO2 NPs can be detected in the cytoplasm of cultured primary neurons imaged by transmission electron microscopy. After TiO2 NP exposure, these neurons also grow shorter axons, which may be related to the detected impeded worm locomotion behavior. Furthermore, anatase TiO2 NPs did not affect the worm’s body length; however, we determined that a concentration of 500 μg/mL of anatase TiO2 NPs reduced the worm population by 50% within 72 h. Notably, rutile TiO2 NPs negatively affect both the body size and worm population. Worms unable to enter the L4 larval stage explain a severe reduction in the worm population at TiO2 NPs LC50/3d. To obtain a better understanding of the cellular mechanisms involved in TiO2 NP i... |
| Databáze: | OpenAIRE |
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