Green Synthesis of Silver Nanoparticle from Datura inoxia Flower Extract and Its Cytotoxic Activity
| Autor: | Wuling Liu, Prabhu Durai, Arulvasu Chinnasamy, Yanmei Li, Radhakrishnan Nagarathnam, Krishnapriya Madhu Varier, Babu Gajendran, Saravanan Rajendran |
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| Rok vydání: | 2019 |
| Předmět: |
010304 chemical physics
biology Chemistry DNA damage Biomedical Engineering Bioengineering 02 engineering and technology 021001 nanoscience & nanotechnology biology.organism_classification 01 natural sciences Silver nanoparticle Datura inoxia Comet assay Biochemistry Cell culture Apoptosis 0103 physical sciences Viability assay Fragmentation (cell biology) 0210 nano-technology |
| Zdroj: | BioNanoScience. 9:564-572 |
| ISSN: | 2191-1649 2191-1630 |
| Popis: | The silver nanoparticles (AgNPs) were synthesized biologically through a green synthesis method using the flower extract of Datura inoxia, a well-known medicinal plant for its antispasmodic, pacifying, pain relief, and for respiratory ailments. The synthesized AgNPs were structurally characterized and the cytotoxic efficiency of the AgNPs on human breast cancer cell line (MCF7) was appraised by cell viability assay, followed by cytomorphological analysis. However, apoptosis initiated by the AgNPs administration was assessed through nuclear fragmentation assay, cell cycle arrest, and comet assay. The synthesized AgNPs inhibited the propagation of cells at an IC50 concentration of 20 μg/mL after 24 h incubation. The AgNPs promoted apoptosis through DNA damage. Thus, this study reveals that the green synthesis is an ecofriendly method for production of AgNPs from Datura inoxia flower extract which provided a powerful anti-proliferative action on MCF7 cell line, suggesting them as a novel chemotherapeutic agent against human breast cancer. |
| Databáze: | OpenAIRE |
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