A large column analog experiment of stable isotope variations during reactive transport: II. Carbon mass balance, microbial community structure and predation

Autor:	Jennifer L. Druhan, Donald J. DePaolo, Eoin L. Brodie, Markus Bill, Cindy H. Wu, Hsiao Chien Lim, Mark E. Conrad, Kenneth H. Williams
Rok vydání:	2014
Předmět:	Total organic carbon Isotopic labeling Nutrient Total inorganic carbon Microbial population biology Geochemistry and Petrology Chemistry Isotopes of carbon Stable isotope ratio Environmental chemistry chemistry.chemical_element Carbon
Zdroj:	Geochimica et Cosmochimica Acta. 124:394-409
ISSN:	0016-7037
DOI:	10.1016/j.gca.2013.08.036
Popis:	Here we report a combined analysis of carbon mass balance based on isotopic labeling and microbiological characterization during organic carbon stimulated bioreduction of a subsurface sediment in a large laboratory column experimental system. This combination of approaches allows quantification of both the cycling of carbon through multiple redox pathways and the associated spatial and temporal evolution of bacterial communities in response to this nutrient source. Carbon isotope mass balance facilitated by the use of 13C-labeled acetate as the electron donor showed evidence for a net loss of sediment organic carbon over the course of the amendment experiment. Furthermore, these data clearly demonstrated a source of isotopically labeled inorganic carbon that was not attributable to primary metabolism by acetate-oxidizing microorganisms. Fluid samples collected weekly over the duration of the 43-day amendment at
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4b6ada2a59d519d3c02bde43a965fbb5 https://doi.org/10.1016/j.gca.2013.08.036 Zobrazit plný text záznamu Full Text from ScienceDirect