| Popis: |
Background Anxiety disorder is a common mental disorder. It is necessary to establish a rapid, stable and specific anxiety model to provide theoretical basis for further research on the pathogenesis of anxiety and drug development. Aim Establish a rapid, stable, specific anxiety model. Methods A single intraperitoneal injection of m-chlorophenylpipera-zine(mCPP) (1, 2, 4 mg/kg) was given to male ICR mice to establish an anxiety model, and the effects of mCPP on anxiety behavior, pain, athletic ability, passive avoidance response ability and depressive behavior of male ICR mice were evaluated. Results A single intraperitoneal injection of mCPP shortened time in open arms and decreased the percentage of time in open arms of mice in elevated plus-maze test, mCPP also shortened center zone distance and reduced the number of entries to central zone in open field test. Moreover, mCPP reduced head-dip counts and increased head-dip latency of mice in hole-board test. After administrated with a single intraperitoneal injection of mCPP for 24h, the mice showed no significant difference for the entry into the light side and the percentage of time in light side of light-dark box test. A single intraperitoneal injection of mCPP had no effects on tail flick latency, rotating time, number of errors and the step-down latency, the immobility time of mice in tail-flick test, rotarod test, step-down test and TST respectively. Conclusion A rapid, stable and specific anxiety mouse model can be constructed by using a single intraperitoneal injection of mCPP. |
