Differential Effects of Nicotine and Aging on Splenocyte Proliferation and the Production of Th1- Versus Th2-Type Cytokines
| Autor: | Lynn Wecker, Herman Friedman, Nora Hallquist, Amal Hakki, Susan Pross |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
medicine.medical_specialty
biology Chemistry Pharmacology General Biochemistry Genetics and Molecular Biology Nicotine chemistry.chemical_compound Endocrinology Nicotinic agonist Immune system Concanavalin A Internal medicine medicine Splenocyte biology.protein Choline Receptor Acetylcholine receptor medicine.drug |
| Zdroj: | Proceedings of the Society for Experimental Biology and Medicine. 224:141-146 |
| ISSN: | 0037-9727 |
| DOI: | 10.1111/j.1525-1373.2000.22412.x |
| Popis: | Nicotine has a multitude of biological actions in the central and peripheral nervous systems where nicotinic acetylcholine receptors are found. Nicotinic acetyl- choline receptors have also been identified on immune cells, but the effects of nico- tine on immune responses are not well characterized. These studies tested the hy- potheses that nicotine has an effect on both T-lymphocyte proliferation and the pro- duction of cytokines by activated T cells, processes that are necessary for effective T-cell-mediated immune responses. In addition, the effects of nicotine on these im- mune responses in aging animals and the effects of nicotine exposure prior to immu- nostimulation were investigated. Murine splenocytes were exposed to nicotine and stimulated with concanavalin A (ConA). The highest concentration of nicotine (128 µg/ml) significantly depressed proliferation of T cells both when nicotine and ConA were added concurrently and when nicotine was added 3 hr prior to ConA. Nicotine, added concurrently with ConA at concentrations between 0.25 and 64 µg/ml, signifi- cantly inhibited the production of IL-10 by splenocytes from young adult mice, whereas the inhibition of production of IL-10 by splenocytes from old mice was sig- nificantly inhibited, but the response was more variable, depending on the nicotine concentration. In contrast, the production of IFN-g by splenocytes from either young adult or old mice was not affected when nicotine (0.016-64 µg/ml) was added concur- rently with ConA. Pre-exposure to 1 µg/ml of nicotine for 3 hr significantly enhanced the production of IFN-g by splenocytes from young adult mice, whereas pre-exposure to 0.016 µg/ml of nicotine tended to but did not significantly enhance IFN- g produc- tion. Nicotine is now being used as an over-the-counter drug by people who differ in age and general immunocompetence. Therefore, the effects of nicotine on immune responses, independent from the effects of the other chemicals found in tobacco, need to be investigated. (P.S.E.B.M. 2000, Vol 224:141-146) |
| Databáze: | OpenAIRE |
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