Screening and Characterization of Antibiotic Producing Organisms from Waste Dump Soil Sample

Autor:	Ruth Asikiya Afunwa, Chigoziri T. Ogu, Ikechukwu B. Awuribeh, Chinenye U. Ezebialu, Uchechukwu G. Nwankwo, Ebere M. Eze
Rok vydání:	2020
Předmět:	Minimum bactericidal concentration medicine.drug_class Providencia stuartii Antibiotics Micrococcus General Medicine Biology Antimicrobial biology.organism_classification Microbiology Ciprofloxacin Minimum inhibitory concentration medicine bacteria Bacteria medicine.drug
Zdroj:	Advances in Microbiology. 10:422-433
ISSN:	2165-3410 2165-3402
Popis:	This study was designed to screen and characterize antibiotic producing bacteria and fungi from soil samples from a waste dump site in Enugu. The molecular characterization of the isolates was performed by using Zymo Research Quick-DNA fungi/bacteria miniprep kit. The bacteria isolates were Providencia stuartii, Providencia alcalifaciens. Bacillus sp., Streptococcus sp., Micrococcus sp., and Staphylococcus sp while Aspergillus fumigatus and Aspergillus niger were fungal isolates. The efficacy of the Crude Extract (CE) of the antibiotics produced was tested against species of Staphylococcus aureus and Escherichia coli. The CE of antibiotics when compared with a conventional antibiotic (Ciprofloxacin) showed lower antimicrobial activity. The minimum inhibitory concentration of the crude extracts ranged between 12.5% and 25% concentrations while the minimum bactericidal concentration ranged from 25% and 50% concentrations. The findings suggest that these organisms have the potential to produce antibiotics and could be harnessed by pharmaceutical industries for the production of newer antibiotics.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4b5a176dfc646381c17bb632be3dead9 https://doi.org/10.4236/aim.2020.109031 Zobrazit plný text záznamu