Isolation and Cultivation of Plasmodium falciparum Using Adult Bovine Serum
| Autor: | Divo Aa, Jensen Jb, Campbell, Vande Waa Ja |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
biology
Horse Plasmodium falciparum biology.organism_classification Isolation (microbiology) Virology Andrology chemistry.chemical_compound chemistry Chloroquine biology.protein medicine Parasite hosting Parasitology Bovine serum albumin Antibody Ecology Evolution Behavior and Systematics Hypoxanthine medicine.drug |
| Zdroj: | The Journal of Parasitology. 71:504 |
| ISSN: | 0022-3395 |
| DOI: | 10.2307/3281546 |
| Popis: | RPMI 1640 medium supplemented with adult bovine serum and hypoxanthine was superior to human serum-supplemented medium for the isolation of new strains of Plasmodium falciparum in Sudan. Similar observations in Indonesia have since confirmed our results. The chloroquine sensitivity of new isolates was identical in either human or bovine serum. Once acclimated to culture conditions P. falciparum strains grew better when using human serum. Erythrocyte-specific antibody present in adult bovine serum slightly inhibited merozoite invasion of uninfected cells. Removal of this cross-reactive antibody from bovine serum increased parasite multiplication to the level obtained in human serum. The continuous cultivation of Plasmodium falciparum was first accomplished using a com- plex medium requiring the addition of 10% hu- man serum (Trager and Jensen, 1976). This re- quirement was reduced to 5% by pooling equal aliquots of serum from a number of different individuals (Divo and Jensen, 1982a). Although beneficial where human serum is readily avail- able, the advantages of using a system which does not require this component are obvious (Divo and Jensen, 1982a). Ideally, a serum-free me- dium is most desirable, although one containing an alternative animal serum represents a signif- icant improvement. To this end other reports have indicated that rabbit (Sax and Rieckmann, 1980), horse (Butcher, 1979), sheep (Chowdhuri and Sivaraman, 1982) or bovine serum (Ifediba and Vanderberg, 1980; Divo and Jensen, 1982a) can replace human serum in continuous P. fal- ciparum cultures. In our hands, and appearing consistently in the literature (Zhengren et al., 1980; Siddiqui, 1981), bovine serum represents the most practical replacement, the others being cost prohibitive or simply unreliable. Our laboratory has demonstrated that RPMI 1640 supplemented with adult bovine serum and hypoxanthine supports continuous culture of P. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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