Interleukin-8-Induced Invasion Assay in Triple-Negative Breast Cancer Cells
| Autor: | Ivana Vancurova, Betsy Deza, Mohammad M. Uddin, Bijaya Gaire |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Chemokine biology Chemistry Bortezomib IκB kinase medicine.disease 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Breast cancer Gentamicin protection assay 030220 oncology & carcinogenesis Cancer cell Cancer research medicine biology.protein Interleukin 8 Triple-negative breast cancer medicine.drug |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071602461 |
| Popis: | The pro-inflammatory and pro-angiogenic chemokine interleukin-8 (IL-8, CXCL8) induces proliferation and invasion of solid tumor cells. In many types of solid cancer, including triple-negative breast cancer (TNBC), the IL-8 expression is induced by proteasome inhibition. In this chapter, we describe a protocol for the analysis of TNBC cell invasion induced by IL-8 in response to proteasome inhibition by bortezomib (BZ). Using this approach, we show that BZ increases the invasion ability of TNBC cells, and that the BZ-increased TNBC cell invasion is suppressed by IκB kinase (IKK) inhibition, which also decreases the IL-8 expression. The experimental protocol includes the cell invasion assay, microscopic evaluation of the invading cells, and quantitative analysis of the obtained images. This protocol should be applicable also for measurement of chemokine-induced tumor cell invasion in other types of cancer cells. |
| Databáze: | OpenAIRE |
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