| Popis: |
In this chapter, we discuss the glutathione cycle in plants and animals and our discovery of cysteine conjugate derivatives in onion via liquid chromatography and tasting (in vivo assay) or by using beta-lyases or bacteria (in vitro assay). Realizing that this strategy could be applied to the discovery of precursors of other sulfur odorants, we used skin microorganisms and sterile human sweat in an in vitro approach to discover [1-(2-hydroxyethyl)-1-methylbutyl]-(L)-cysteinylglycine (Cys-Gly-S-conjugate), the precursor of a human axillary odorant, (R/S)-3-methyl-3-sulfanylhexan-1-ol. The diversity of cysteine-S-conjugates in plants was illustrated by analysis of Allium caepa L. |
