Reactivation of an immobilized enzyme reactor for the determination of acetylcholinesterase inhibitors Flow injection determination of paraoxon
| Autor: | Alan Townshend, Cándido García de María, Teresa Manzano Muñoz |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
chemistry.chemical_classification
Detection limit Chromatography Paraoxon Immobilized enzyme biology medicine.diagnostic_test Biochemistry Acetylcholinesterase Analytical Chemistry chemistry.chemical_compound Enzyme chemistry Bromide Enzyme inhibitor Spectrophotometry medicine biology.protein Environmental Chemistry Spectroscopy medicine.drug |
| Zdroj: | Analytica Chimica Acta. 295:287-296 |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/0003-2670(94)80234-3 |
| Popis: | A stopped-flow/flow-injection procedure is proposed for the spectrophotometric determination of paraoxon based on the inhibition of immobilized acetylcholinesterase. The irreversibly inhibited enzyme is reactivated with 1,1′-trimethylene-bis(4-formylpyridinium bromide) dioxime. The proposed procedure has a detection limit of 4 × 10−9 M, a linear calibration range of 9 × 10−9−5 × 10−7 M, a R.S.D. of 1.6% (n = 10) at 5.0 × 10−8 M and a sample throughput frequency of 10 h−1. The enzyme reactor can be used for more than one thousand inhibition-reactivation cycles. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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