Presence of alternative complement pathway-inhibiting protein (ACPIP) from silkworm (Bombyx mori): changes in activity at various stages of fifth larval stadium

Autor: Tetsuo Hiyama, Kunikatsu Hamano, Yuriko Fujikura, Jun Saito, Takashi Takahashi, Zhang Yi Cheng, Yasutaka Sekijima
Rok vydání: 2003
Předmět:
Zdroj: Applied Entomology and Zoology. 38:163-166
ISSN: 1347-605X
0003-6862
DOI: 10.1303/aez.2003.163
Popis: The activities of human alternative complement pathway-inhibiting protein (ACPIP) were examined in silkworm (Bombyx mori) larvae at various developmental stages, using both hemolysis-inhibition plate assay and immunoblotting analysis. Significant activities were detected in the hemolymph ranging from the fourth molt to prepupal stages, the highest being those on the 6th to 9th days of the fifth stadium. Although the activity was also detected at the pupal stage, it was only 1/16 of the maximum. These results suggested that ACPIP in the silkworm hemolymph might play a physiological role at the larval stage rather than at the pupae stage. ACPIP-like activities were also detected in hemolymph from final stadium larvae of Antheraea pernyi, Hense convolvuli, Antheraea yamamai, and Rhodinia fugax.
Databáze: OpenAIRE