Abstract 1105: MicroRNAs and their targets genes promote cisplatin resistance in epithelial ovarian cancer cells

Autor: Pablo E. Vivas-Mejia, Fatma Valiyeva, Ileabett M. Echevarria Vargas
Rok vydání: 2012
Předmět:
Zdroj: Cancer Research. 72:1105-1105
ISSN: 1538-7445
0008-5472
DOI: 10.1158/1538-7445.am2012-1105
Popis: MicroRNAs (miRNAs) are 21-25 nucleotides regulatory involved in silencing mRNAs in a sequence specific manner. Accumulated data has established the central role of various microRNAs in tumor initiation and progression. Furthermore, recent studies revealed that microRNAs participate in the resistance of cancer cells to different chemotherapeutic agents. In ovarian carcinoma, the fifth cause of cancer deaths in US, it has been shown that several miRNAs contribute to drug resistance. However, the role of these miRNAs in the cisplatin resistance of ovarian cancer cells is unclear. In a microarray study, we found that miR-21, miR27b and miR-622 were upregulated in the A2780CP20 cisplatin resistant ovarian cancer cells compared with the A2780PAR cells, which are sensitive to cisplatin treatment. Real-time PCR studies confirmed these findings. Opposite, the c-Jun transcription factor was upregulated in this microarray analysis. Then, we used TargetScan and miRBase tools to identify miR-21, miR-27b and miR-622- target genes. Interestingly, several of these miRNA-target genes were identified as downregulated in our microarray study. These results were corroborated by Western blot analysis. Moreover, cell survival experiments in the presence of a miR-21 mimic, made A2780PAR cells more resistant to cisplatin treatment. These results indicate that dysregulation of various miRNAs and their target genes contribute to the cisplatin resistance of ovarian cancer cells. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 1105. doi:1538-7445.AM2012-1105
Databáze: OpenAIRE