Isolation and Characterization of Innate Lymphoid Cells within the Murine Tumor Microenvironment
| Autor: | Megan Payne, Warren Patterson, Grace Mallett, Shoba Amarnath |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Tumor microenvironment Intracellular parasite Melanoma Innate lymphoid cell Biology medicine.disease body regions 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Murine tumor Immunity 030220 oncology & carcinogenesis Extracellular medicine Cancer research skin and connective tissue diseases Tissue homeostasis |
| Zdroj: | Methods in Molecular Biology ISBN: 9781071603376 |
| Popis: | Innate lymphoid cells (ILCs) are important for both tissue immunity and tissue homeostasis. They are classified into three groups: Group 1 ILCs include NK cells, which are important in eliciting immunity against intracellular pathogens; group 2 ILCs protect against parasitic helminths; and group 3 ILCs protect against extracellular pathogens. The role of ILCs in cancer immunity remains unclear. In this chapter, we discuss methods for isolating and characterizing tumor-infiltrating ILC subsets within the tumor microenvironment in an experimental murine model of B16 melanoma. The chapter also highlights the expression of PD-1 on the various ILC subsets within the tumor microenvironment. |
| Databáze: | OpenAIRE |
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