Surface Plasmon Resonance Aptamer Biosensor for Discriminating Pathogenic Bacteria Vibrio parahaemolyticus
| Autor: | Sung-Jin Cho, Sung-Keun Rhee, Sang-Yong Kim, Simranjeet Singh Sekhon, Moon-Jong Lee, Kyeong-Ah Lee, Janet Han, Jiho Min, Lyon Lee, Jung Ho Ko, Yang-Hoon Kim, Ji-Young Ahn |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Materials science Aptamer Biomedical Engineering Bioengineering medicine.disease_cause 01 natural sciences Microbiology 03 medical and health sciences Listeria monocytogenes medicine General Materials Science Escherichia coli biology Vibrio parahaemolyticus 010401 analytical chemistry Pathogenic bacteria General Chemistry biochemical phenomena metabolism and nutrition Condensed Matter Physics biology.organism_classification Vibrio 0104 chemical sciences 030104 developmental biology SELEX Aptamer Technique bacteria Systematic evolution of ligands by exponential enrichment |
| Zdroj: | Journal of Nanoscience and Nanotechnology. 18:1599-1605 |
| ISSN: | 1533-4880 |
| DOI: | 10.1166/jnn.2018.14212 |
| Popis: | In this paper, whole-bacteria SELEX (WB-SELEX) strategy was adopted to isolate specific aptamers against Vibrio parahaemolyticus. Round selection for V. parahaemolyticus was conducted 11 rounds, including two negative selection rounds. It was determined through real-time PCR amplification and post-SELEX experiment. The selected aptmers had high binding property and specificity to V. parahaemolyticus. Of 28 aptamers tested, VPCA-apta#1 had the highest binding affinity compared to other aptamer candidates obtained. To detect V. parahaemolyticus, aptamer based SPR biosensor platform was constructed and pathogenic bacteria sensing was conducted in two steps. The first step was to construct 5'-biotinylated VPCA-apta#1 binding probe. The second step was to incubate V. parahaemolyticus and test microbes in functionalized SA sensor chip in parallel. Our platform showed significant activity for detecting and discriminating V. parahaemolyticus from other enteric species such as Escherichia coli, Listeria monocytogenes, Sigella sonnei, and Vibrio fischeri. This is the first report on the use of whole-SELEX to isolate DNA aptamers specific for V. parahaemolyticus. We demonstrated the feasibility of using aptamer platform for the detection of V. parahaemolyticus in various food supplies. It might be used in multiple points of care for diagnosing Vibriosis. |
| Databáze: | OpenAIRE |
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