| Autor: |
José Paulo Domingues, Miguel Morgado, Susana F. Silva, Ana Batista, Maria João Quadrado |
| Rok vydání: |
2015 |
| Předmět: |
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| Zdroj: |
2015 IEEE 4th Portuguese Meeting on Bioengineering (ENBENG). |
| DOI: |
10.1109/enbeng.2015.7088878 |
| Popis: |
Assessing corneal metabolism may provide clinicians a tool for diagnosing corneal cells dysfunctions prior to its pathological expression. Flavin adenine dinucleotide (FAD), a metabolic co-factor, exhibits two lifetime components (long and short) upon blue light excitation. Due to that, fluorescence lifetime imaging microscopy (FLIM) may provide a method to evaluate corneal cells metabolism non-invasively. We are developing a single-photon, time-gated fluorescence lifetime microscope for in vivo corneal imaging using structured illumination to improve optical sectioning. Single-photon imaging is provided by a picosecond diode laser with emission at 443nm. Structured illumination is implemented by modulating the laser light through a Digital Micromirror Device (DMD). The fluorescence imaging acquisition is based on an ultrafast time-gated intensified CCD camera operating with gates down to 200ps. We present preliminary data regarding the timing and optical performance of the microscope. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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