A Cell Factory of a Fungicolous Fungus Calcarisporium arbuscula for Efficient Production of Natural Products
| Autor: | You-Min Ying, Yong-Quan Li, Jia-Hui Yu, Zha-Jun Zhan, Li-She Gan, Qing-Wei Zhao, Xu-Ming Mao, Wen-Ju Li, Fei Cao, Jin-Tao Cheng, Xin-Ai Chen, Chen-Fan Sun |
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| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
0303 health sciences Aspergillus Calcarisporium arbuscula biology Host (biology) Mutant Biomedical Engineering General Medicine Fungus biology.organism_classification 01 natural sciences Biochemistry Genetics and Molecular Biology (miscellaneous) 03 medical and health sciences chemistry.chemical_compound chemistry 010608 biotechnology Penicillium Botany Mycotoxin Gene 030304 developmental biology |
| Zdroj: | ACS Synthetic Biology. 10:698-706 |
| ISSN: | 2161-5063 |
| DOI: | 10.1021/acssynbio.0c00371 |
| Popis: | Fungal natural products are rich sources of clinical drugs. Particularly, the fungicolous fungi have a large number of biosynthetic gene clusters (BGCs) to produce numerous bioactive natural products, but most BGCs are silent in the laboratory. We have shown that a fungicolous fungus Calcarisporium arbuscula NRRL 3705 predominantly produces the highly reduced polyketide-type mycotoxins aurovertins. Here after evaluation of the aurovertin-null mutant ΔaurA as an efficient host, we further screened two strong promoters aurBp and A07068p based on RNA-Seq, and successfully activated an endogenous gene cluster from C. arbuscula as well as three additional exogenous BGCs from other fungi to produce polyketide-type natural products. Thus, we showed an efficient expression system from the fungicolous fungus C. arbuscula, which will be highly beneficial and complementary to the conventional Aspergillus and Penicillium fungal cell factories, and provides a useful toolkit for genome-wide mining of bioactive natural products from fungicolous fungi. |
| Databáze: | OpenAIRE |
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