Experimental proof for a signal peptidase I like activity in Mycoplasma pneumoniae, but absence of a gene encoding a conserved bacterial type I SPase

Autor:	Thomas Ruppert, Armin Bosserhoff, Richard Herrmann, Ina Catrein
Rok vydání:	2005
Předmět:	chemistry.chemical_classification Signal peptide Whole genome sequencing Signal peptidase Cell Biology Biology Cleavage (embryo) Biochemistry Amino acid Gene product chemistry Molecular Biology Signal peptide peptidase Gene
Zdroj:	FEBS Journal. 272:2892-2900
ISSN:	1742-4658 1742-464X
DOI:	10.1111/j.1742-4658.2005.04710.x
Popis:	Although the annotation of the complete genome sequence of Mycoplasma pneumoniae did not reveal a bacterial type I signal peptidase (SPase I) we showed experimentally that such an activity must exist in this bacterium, by determining the N-terminus of the N-terminal gene product P40 of MPN142, formerly called ORF6 gene. Combining mass spectrometry with a method for sulfonating specifically the free amino terminal group of proteins, the cleavage site for a typical signal peptide was located between amino acids 25 and 26 of the P40 precursor protein. The experimental results were in agreement with the cleavage site predicted by computational methods providing experimental confirmation for these theoretical analyses.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::4b212f926346a57cca7dd9409a283b08 https://doi.org/10.1111/j.1742-4658.2005.04710.x Zobrazit plný text záznamu Plný text ve formátu PDF