Perfusion of rabbit kidneys with glycerol solutions at 5 °C
| Autor: | M.C. Wusteman, D.E. Pegg |
|---|---|
| Rok vydání: | 1977 |
| Předmět: |
Chromatography
Cryoprotectant Sodium chemistry.chemical_element Renal function General Medicine General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound Dextran chemistry Biochemistry Renal physiology Glycerol medicine Mannitol General Agricultural and Biological Sciences Perfusion medicine.drug |
| Zdroj: | Cryobiology. 14:168-178 |
| ISSN: | 0011-2240 |
| DOI: | 10.1016/0011-2240(77)90137-7 |
| Popis: | The long-term preservation of whole organs will almost certainly require the use of subzero temperatures and cryoprotectants. An essential part of such a technique is the ability to add a cryoprotectant in adequate concentration and subsequently to remove it without damage to the organ. In this study rabbit kidneys have been perfused with solutions containing 3% dextran and 2 m glycerol at 5 °C, and their function has been measured after removal of the glycerol. The assay technique involved the measurement of glomerular filtration rate, protein leakage, and tubular reabsorption of sodium and glucose. The results indicate that the inclusion in the perfusate of an impermeant solute (mannitol) and limitation of the rate of change of glycerol concentration (to 30 mm min−1) permits rabbit kidneys to retain a degree of function similar to that found in perfused control kidneys, although somewhat reduced in comparison with freshly isolated kidneys. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|