Long Noncoding RNA NEAT1 Upregulates Survivin and Facilitates Gallbladder Cancer Progression by Sponging microRNA-335
| Autor: | Lei Yin, Ningjia Shen, Facai Yang, Anqi Duan, Bin Yi, Zhiyuan Bo, Yinghe Qiu, Bin Zhu, Jingdong Li, Zhaohui Tang |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Gene knockdown Cell growth Cancer Biology medicine.disease Long non-coding RNA Blot 03 medical and health sciences 030104 developmental biology 0302 clinical medicine Oncology Downregulation and upregulation 030220 oncology & carcinogenesis microRNA Survivin Cancer research medicine Pharmacology (medical) |
| Zdroj: | OncoTargets and Therapy. 13:2357-2367 |
| ISSN: | 1178-6930 |
| Popis: | Background Gallbladder cancer (GBC) is the most common cancer of the biliary tract, but molecularly targeted therapies are not available for GBC. Loss of microRNA (miR)-335 expression may be a useful predictor of clinical outcomes and the reversal of its loss of expression may be a useful treatment strategy for GBC. In this study, we investigated whether a long noncoding RNA, nuclear paraspeckle assembly transcript 1 (NEAT1) sponges miR-335 in GBC cells. Materials and methods Quantitative reverse transcription-polymerase chain reaction (qRT-PCR), Western blotting, and immunohistochemistry were used to determine the expression of miR-335; NEAT1; survivin; and Ki67 in GBC cell lines (GBC-SD and SGC-996) and tissue samples from patients (n = 25). Cell Counting Kit-8, colony-formation, and Transwell migration and invasion assays were performed to measure cell proliferation, migration, and invasion. Bioinformatic analysis and dual-luciferase reporter assays were utilized to analyze correlativity. Results miR-335 overexpression resulted in inhibition of GBC cell proliferation and invasion. In addition, knockdown of NEAT1 resulted in downregulation of survivin expression. As NEAT1 competitively "sponges" miR-335, NEAT1 knockdown resulted in inhibited GBC cell proliferation and invasion in vitro and GBC tumor growth in vivo. Furthermore, NEAT1 was found to be upregulated in GBC samples, and its expression was inversely correlated with miR-335 levels, but positively correlated with survivin levels. Conclusion These findings indicate that NEAT1 promotes survivin expression by functioning as a competitive endogenous RNA for miR-335 in GBC cells; thus, we have identified a potential biomarker and target for GBC diagnosis and therapy. |
| Databáze: | OpenAIRE |
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