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Publisher Summary This chapter discusses the measurement of in vivo leucocyte chemotaxis mediated by Fc-binding proteins. The in vivo measurement of vectoral, chemotactic migration has been well documented. These in vitro methods have defined not only the existence of chemotactic compounds but also the presence of membrane-bound receptors for these agents on responding (migrating) cells. The phenotype of these cells has also been characterized. The leucocyte migrational response in vivo may be because of (1) the direct chemotactic response to the agent itself or (2) the generation of a physiological, chemotactic factor(s) in vivo . Apart from the influence of chemotactic agents, the response can also be affected by various local and systemic inhibitory or amplifying systems. The major advantages of this method are its simplicity and its accommodation of large numbers of samples. This enables multiple time points and concentrations to be studied and facilitates the use of multiple animals in each experimental protocol. This procedure is, therefore, of value for the rapid evaluation of the ability of bacterial Fc-binding proteins to mediate immediate or delayed hypersensitivity responses in vivo . |
