| Popis: |
Before a new type of mammalian cells or medium is introduced to large-scale production, it is important to know how the cells will respond to the new shear conditions. Accurate quantification of the optimum process conditions or parameter ranges at this early stage is highly desirable, resulting in a significant reduction of the large-scale optimization work. Since variables related to shear are of paramount importance at production scale, a simple and efficient method for shear testing at small scale is required. Such a test would be used for: new cell line characterizations evaluation the protective effect of new medium formulations prediction the effect of agitation, temperature, and other fermentation conditions on the shear damage of cells in the production fermentor. |
