Headspace gas-chromatographic determination of formaldehyde in urine
| Autor: | Efimova Nv, A. N. Alekseenko, Rukavishnikov Vs, O. M. Zhurba |
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| Rok vydání: | 2017 |
| Předmět: |
Detection limit
education.field_of_study Chromatography 010401 analytical chemistry Population Formaldehyde 02 engineering and technology Urine 021001 nanoscience & nanotechnology Oxime 01 natural sciences 0104 chemical sciences Analytical Chemistry law.invention chemistry.chemical_compound Hydroxylamine chemistry law Flame ionization detector Gas chromatography 0210 nano-technology education |
| Zdroj: | Journal of Analytical Chemistry. 72:83-86 |
| ISSN: | 1608-3199 1061-9348 |
| Popis: | A method is proposed for determining formaldehyde by headspace gas chromatography using an o-(2,3,4,5,6-pentafluorobenzyl)hydroxylamine (PFBHA) derivatizing agent. Formaldehyde in urine was derivatized to o-(pentafluorobenzyl)oxime and extracted by heating an urine sample with PFBHA in a sealed vial. Gas-chromatographic analysis of the headspace was performed in the temperature-programmed mode using an HP-5 capillary column with a flame ionization detector. The limit of detection is 3.5 μg/L. The accuracy of the procedure, equivalent to an extended relative uncertainty, does not exceed 21%. The examination of the procedure using urine samples of the population of the Irkutsk region has shown that the regional reference levels of formaldehyde concentration are in the range 44–83 μg/L. |
| Databáze: | OpenAIRE |
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