Real time visualization and characterization of platelet deposition under flow onto clinically relevant opaque surfaces
| Autor: | James F. Antaki, Marina V. Kameneva, William R. Wagner, Megan A. Jamiolkowski, Joshua R. Woolley |
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| Rok vydání: | 2014 |
| Předmět: |
chemistry.chemical_classification
Materials science Biocompatibility Opacity Zirconia Toughened Alumina Metals and Alloys Biomedical Engineering Titanium alloy Nanotechnology Polymer Biomaterials chemistry Ceramics and Composites Platelet Cubic zirconia Yttria-stabilized zirconia Biomedical engineering |
| Zdroj: | Journal of Biomedical Materials Research Part A. 103:1303-1311 |
| ISSN: | 1549-3296 |
| DOI: | 10.1002/jbm.a.35202 |
| Popis: | Although the thrombogenic nature of the surfaces of cardiovascular devices is an important aspect of blood biocompatibility, few studies have examined platelet deposition onto opaque materials used for these devices in real time. This is particularly true for the metallic surfaces used in current ventricular assist devices (VADs). Using hemoglobin depleted red blood cells (RBC ghosts) and long working distance optics to visualize platelet deposition, we sought to perform such an evaluation. Fluorescently labeled platelets mixed with human RBC ghosts were perfused across six opaque materials (a titanium alloy (Ti6Al4V), silicon carbide (SiC), alumina (Al2O3), 2-methacryloyloxyethyl phosphorylcholine polymer coated Ti6Al4V (MPC-Ti6Al4V), yttria partially stabilized zirconia (YZTP), and zirconia toughened alumina (ZTA)) for 5 min at wall shear rates of 400 and 1000 s−1. Ti6Al4V had significantly increased platelet deposition relative to MPC-Ti6Al4V, Al2O3, YZTP, and ZTA at both wall shear rates (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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