Singlet Oxygen Sensor Green®: Photochemical Behavior in Solution and in a Mammalian Cell
| Autor: | Brian W. Pedersen, Jacob Arnbjerg, Frances H. Blaikie, Marianne Glasius, Anita Gollmer, Peter R. Ogilby, Thomas Breitenbach, Kim Daasbjerg |
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| Rok vydání: | 2011 |
| Předmět: |
inorganic chemicals
chemistry.chemical_classification Reactive oxygen species Singlet molecular oxygen Singlet oxygen General Medicine Photochemistry Biochemistry Fluorescence chemistry.chemical_compound chemistry Yield (chemistry) Mammalian cell biological sciences polycyclic compounds Photosensitizer Physical and Theoretical Chemistry Spectrum analysis |
| Zdroj: | Photochemistry and Photobiology. 87:671-679 |
| ISSN: | 0031-8655 |
| DOI: | 10.1111/j.1751-1097.2011.00900.x |
| Popis: | The development of efficient and selective luminescent probes for reactive oxygen species, particularly for singlet molecular oxygen, is currently of great importance. In this study, the photochemical behavior of Singlet Oxygen Sensor Green(®) (SOSG), a commercially available fluorescent probe for singlet oxygen, was examined. Despite published claims to the contrary, the data presented herein indicate that SOSG can, in fact, be incorporated into a living mammalian cell. However, for a number of reasons, caution must be exercised when using SOSG. First, it is shown that the immediate product of the reaction between SOSG and singlet oxygen is, itself, an efficient singlet oxygen photosensitizer. Second, SOSG appears to efficiently bind to proteins which, in turn, can influence uptake by a cell as well as behavior in the cell. As such, incorrect use of SOSG can yield misleading data on yields of photosensitized singlet oxygen production, and can also lead to photooxygenation-dependent adverse effects in the system being investigated. |
| Databáze: | OpenAIRE |
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